MUSCULAR INTERACTION PROJECT

Muscular Interactome Project

Explore the Muscular Protein Interaction Map with PIMRider (free registration required):

  1. The original dataset corresponding to the article by Blandin et al. is available here
  2. An updated dataset is now available : i) the confidence scores for 125 interactions were updated; ii) literature protein interactions reported in at least two different articles were incorporated (retrieved by interrogating the public databases IntAct, Mint, Biogrid and HPRD)

The Muscular Protein Interaction Map

The complexity of the skeletal muscle and the identification of numerous human disease-causing mutations in its constitutive proteins make it an interesting tissue for proteomic studies aimed at understanding functional relationships of interacting proteins in both health and diseases.

Isabelle Richard's team at Généthon (Evry, France) commissioned a proteome-scale map of protein-protein interactions centered on proteins involved in limb-girdle muscular dystrophies (LGMD) to Hybrigenics (Blandin et al., 2013). LGMD is a group of more than 20 different neuromuscular disorders that principally affect the proximal pelvic and shoulder girdle muscles.

The resulting interaction network incorporates 1018 proteins connected by 1492 direct binary interactions and includes 1420 novel protein-protein interactions. Computational, experimental and literature-based analyses were performed to assess the overall quality of this network. Interestingly, LGMD proteins were shown to be highly interconnected, in particular indirectly through sarcomeric proteins. In-depth mining of the LGMD-centered interactome identified new candidate genes for orphan LGMDs and other neuromuscular disorders. The data also suggest the existence of functional links between LGMD2B/dysferlin and gene regulation, between LGMD2C/gamma-sarcoglycan and energy control and between LGMD2G/telethonin and maintenance of genome integrity.

Reference:
Blandin G, Marchand S, Charton K, Danièle N, Gicquel E, Boucheteil JB, Bentaib A, Barrault L, Stockholm D, Bartoli M and Richard I. A human skeletal muscle interactome centered on proteins involved in muscular dystrophies: LGMD interactome (2013), Skeletal Muscle 3(1):3.

 
© 2012 hybrigenics